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GC Method Development Video Training Course

Starts January 16th 2020.
4 video sessions - each session is between
1- 2 hours long.

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Method development in the modern GC laboratory is challenging but can be approached very logically and is hugely rewarding when a fit for purpose method is produced. This course provides you with the underpinning knowledge to begin your GC method development career.

This course has been designed for those who are experience in GC and who are now looking to develop their experience and understanding of method development. During the course we will assume that you are familiar with the basic theories of gas phase chromatographic separations, but we’ll also revisit some theory to underpin the teaching.

Course Delivery & Duration

  • 4 video sessions - each session is between 1- 2 hours long
  • Tutor supported with access to our chromatography experts
  • Supporting training material
  • Course assessment with certificate

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Practical LC-MS - Video Boot Camp
From instrument setup to MS/MS transitions

10 part video training course

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Working at a real LC-MS system and with real data, our technical team methodically take you through the practical process of LC-MS; from basic instrument setup, to mobile phase and signal optimization, and up to complex MS/MS experiments.

We demonstrate each stage while giving a clear explanation of the background science which underpins this practical approache, giving the user a detailed understanding of all the important aspects of LC-MS which are critical for reliable day-to-day running of this amazing technique.

During the training we will look at the following topics:

  • Introduction
  • Infusion
  • Basic Signal
  • Adducts
  • Signal Optimization and Tuning
  • CID
  • MS/MS
  • Isotopes
  • SRM
  • Conclusion

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Workflows for Released N-Glycan Analysis and Sialic Acid Quantitation of Biotherapeutics  
HPLC and GC Troubleshooting Videos

Native and Denaturing Mode Size Exclusion Analysis of Biotherapeutics

FREE CHROMacademy Essential Guide Webcast.

This webcast will focus on the versatile use of SEC in analyzing biotherapeutics. The talk will introduce the Agilent SEC column portfolio, including the AdvanceBio SEC columns with sub-2- µm particle technology, consideration of various pore sizes, and selecting an appropriate column for SEC analysis of different sample modalities. We will also demonstrate SEC-MS applications under both denaturing and native mode for biotherapeutic analyses.

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Time limited free access to our Lite members. In 3 simple steps we can help you overcome your instrument, separation and quantitation issues

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HPLC and GC Troubleshooting Videos
HPLC and GC Troubleshooting Videos

HPLC Column Dimensions - Quick Guide

HPLC columns are manufactured in a variety of different internal diameter and length combinations, as well as having an assortment of particle sizes. 

HPLC column dimensions will affect the efficiency sensitivity, and speed of an analysis. The choice of column dimensions will depend on the chromatographic application; analytical, semi-preparative, preparative, number of analytes in the mixture etc.  However, the dimensions can also be altered to improve chromatography by achieving more efficient, sensitive, and faster analyses.  The primary column dimensions of particle size, length, and internal diameter will be discussed and their effect on chromatographic separations demonstrated, providing some practical insight into how to select the best column dimensions for a particular application...

HPLC Column Dimensions »

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The Why, What, and How of Headspace GC

The first question to ask is, why would headspace GC be a good sampling technique?

Headspace sampling is excellent for the qualitative or quantitative analysis of volatile species in samples that can be efficiently partitioned into the headspace gas volume from either a liquid or solid matrix.  It is also a good technique for the analysis of samples where the entire sample should not be injected into the GC instrument (i.e. dirty samples).  Headspace sampling is also particularly amenable to the analysis of trace levels of analytes.  

Typical examples of headspace analyses include...

View Headspace GC Quick Guide »

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